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The only way to know if hormones are in balance is to test them. Therefore, it’s vital to partner with a lab that understands subtle differences between testing in different sample types and has both the experience and technology to deliver the most accurate results.


The only way to know if hormones are in balance is to test them. Therefore, it’s vital to partner with a lab that understands subtle differences between testing in different sample types and has both the experience and technology to deliver the most accurate results.

Revelry offers testing in 4 different sample types so you can choose the option that is best for you and your patient’s needs to achieve optimal clinical outcomes.

Our Testing Methodologies:

Saliva Testing identifies bioavailable hormone levels – the active quantity that’s free to move into body tissue. Its non-invasive nature also makes it the gold standard for tests requiring multiple collections, such as diurnal cortisol.

Suitable for:

  • Assessing “free” (unbound to carrier proteins) hormone levels
  • Monitoring hormone replacement given orally, topically, vaginally or via pellets
  • Collecting multiple samples during a day, e.g., determining diurnal cortisol levels for adrenal stress assessment

Not suitable for:

  • Monitoring sublingual/troche hormone replacement
  • Patients with dry mouth, e.g., due to Sjögren’s Syndrome

Dried Blood Spot Testing is ideal for measuring hormones and other analytes such as insulin, blood lipids, Vitamin D, thyroid hormones, and elements like lead and magnesium. Blood spot testing gives results equivalent to serum but with distinct advantages over serum testing for monitoring topical and vaginal hormone supplementation.

Suitable for:

  • Assessing total circulating hormone levels (free plus protein-bound)
  • Patients with dry mouth and/or children who may have difficulty collecting saliva
  • Monitoring hormone replacement therapy (oral, sublingual, pellet)
  • Assessing thyroid health, fertility parameters, and cardiometabolic risk factors

Dried Blood Spot testing is superior to Serum for:

  • Assessing interstitial tissue/capillary hormone levels reflecting topical or vaginal hormone supplementation
  • Toxic and nutritional elements requiring a whole blood sample
  • Self-collection of sample at home at a time convenient to the patient and avoiding a trip to the phlebotomist

Dried Urine Testing is the patient-friendly, discreet, simple alternative to all-day urine collection in a jug. Only 4 samples in one day are needed to assess adrenal and sex steroid hormone metabolic by-products and neurotransmitters, while 2 samples accurately determine 24-hour excretion of essential elements like iodine and selenium, and toxic heavy metals like arsenic and cadmium.

Suitable for:

  • Measuring steroid hormone metabolites, e.g., for breast cancer risk assessment
  • Determining toxic element exposure and iodine/selenium sufficiency for thyroid health
  • Determining diurnal cortisol production at 4 time points for stress assessment
  • Assessing nocturnal and diurnal melatonin production

Not suitable for:

  • Monitoring topical or intravaginal hormone replacement therapy

Serum Testingis a broadly accepted method for measuring hormones and other analytes that circulate in the bloodstream. It cannot be used to assess analytes that require a whole blood sample, e.g., HbA1c or lead, for which dried blood spot should be used instead.

Suitable for:

  • Assessing total circulating endogenous hormone levels (free plus protein-bound)
  • Monitoring hormone replacement therapy (sublingual, patch, pellet)
  • Assessing thyroid health
  • Assessing analytes that cannot be determined in dried blood spot, e.g., ferritin

Not suitable for:

  • Monitoring topical hormone replacement therapy (underestimates tissue uptake)
  • Monitoring oral hormone replacement therapy (circulating metabolites interfere with immunoassays)
  • Assessing analytes that require a whole blood sample, e.g., HbA1c or blood lead

Sample Test

  • Saliva
  • Blood Spot
  • Dried Urine
  • Serum
  • Comparing Sample Types
  • How to Collect Your Sample
  • Revelry Test Reports

Test Specialties


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See our Samples Types


Dried Blood Spot

Dried Urine


ZRT test kits offer a simple and convenient home collection of saliva. dried blood spot or dried urine samples.

The resources we otter make sample collection easy – whether you prefer watching a video or following detailed instructions. We also provide guidance for which patients will benefit from our testing and how to ensure they collect usable samples.

Who to Test

Candidates for hormone testing include men and women who exhibit symptoms including:

  • Hot flashes
  • Night sweats
  • Foggy thinking
  • Sleep disturbances
  • Low libido / decreased sex drive
  • Vaginal dryness
  • Acne
  • Increased body / facial hair
  • Heavy or irregular menses
  • PMS / PMDD
  • Fibrocystic breasts
  • Breast cancer
  • Infertility
  • PCOS
  • Chronic stress
  • Chronic fatigue
  • Weight gain, especially around the waist
  • Heart palpitations
  • Dry skin / brittle nails
  • Cold hands and feet

Unraveling the Contusion over Saliva, Blood Spot. Serum & Urine Testing for Steroid Hormone Levels

Today’s health care practitioners face the challenge of helping patients cope with hormone imbalance. Whether that’s women suffering from hot flashes and night sweats or men troubled by symptoms of low testosterone. accurate testing is the best way to make sure hormone dosing is optimal.

Matching the type of testing with the type of supplementation has a great Impact on the clinical usefulness of hormone assessments. If you’re measuring with the wrong method, tissue uptake of supplemented hormone may not be accurately reflected In test results – leading to Inappropriate dosing.

Serum vs. Blood Spot

While testing endogenous hormones in serum or blood spot reveals the same levels, assessing topical hormone supplementation with serum testing grossly underestimates the amount of hormone being delivered to tissues. Blood spot tests blood in the capillary beds (arterial/venous/lymphatic) of the finger, and thus better reflects tissue hormone levels.

Serum vs. Saliva

With saliva measuring the bioavailable (non-protein-bound) fraction of circulating hormones that can freely diffuse into tissues, it provides a more accurate assessment of topical hormone supplementation than serum. Serum levels do not rise significantly after topical dosing. By contrast, saliva levels do – reflecting tissue delivery of the topically applied hormone.

Blood Spot vs. Saliva

Blood spot and saliva are highly accurate methods for assessing oral, topical, vaginal, injectable and pellet hormone delivery. However, saliva is not accurate for troche or sublingual hormone therapies because these deliver high amounts of hormone locally to the salivary glands – giving a false-high determination of whole body exposure to the supplemented hormone.

Blood Spot / Saliva vs. Urine

Urine testing cannot accurately assess topical or oral medications – as it is not reflective of tissue uptake – and may show no uptake with topical or extremely high levels with oral medications. Urine testing is not recommended for assessing vaginal hormone delivery as there is a high risk of contamination of the urine sample leading to false-high results. Blood spot or saliva testing provide the best assessment of oral, topical and vaginal hormone supplementation.

Saliva vs. Urine

Urinary hormone testing is the only way to see how the body is metabolizing hormones. Both saliva and urine can be used for measuring diurnal cortisol levels; but urinary free cortisol output reflects an average of the time since the previous urine void (hours), while saliva provides an instantaneous assessment at the time saliva was collected (minutes).

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    Our Locations

    Institute of Neuromuscular Medicine

    4300 Marseilles St Detroit, MI 48224

    Eastpointe Family Physicians

    24901 Kelly Rd Eastpointe, MI 48021

    MedSports Vantage

    10381 Citation Drive Suite #250, Brighton, MI 48116